Date of Completion

2025

Document Type

Research Project

Degree Name

Grade 12

Keywords

Gnetum gnemon L., Bacillus cereus, Pseudomonas aeruginosa, Kirby- Bauer Disk Diffusion Method, plant-based antibiotics

Abstract

This study explored the antibacterial effect of the ethanolic leaf extract of Gnetum gnemon L. (Bago) against Bacillus cereus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative). The goal was to find out if different extract concentrations (25%, 50%, 75%, and 100%) could stop the growth of these foodborne bacteria. The experiment was done at De La Salle Medical and Health Sciences Institute using the Kirby-Bauer Disk Diffusion Method. Results showed that the extract caused only weak inhibition against Bacillus cereus, with zones of inhibition between 6.50 mm and 6.93 mm. For Pseudomonas aeruginosa, no inhibition was seen at any concentration, showing that the extract had no effect. These findings suggest that the antibacterial compounds in the leaves were not strong enough to fight the tested bacteria. Compared to standard antibiotics like vancomycin and ciprofloxacin, the extract’s effect was very minimal. Overall, the study shows that Gnetum gnemon L. leaves have limited antibacterial properties. Future research is recommended to test other parts of the plant or different extraction methods that may produce stronger antibacterial effects.

First Advisor

Myra Michelle Mojica, LPT, MSc

Download

Share

COinS