Date of Completion

2024

Document Type

Research Project

Degree Name

Grade 12

Keywords

Staphylococcus Aureus, Escherichia Coli

Abstract

Honey is a complex supersaturated sugar solution high in vitamins and sugar, making it valuable in food, medicine, and other nutraceutical products. Its utilization as a broad-spectrum antibacterial agent can prevent antibiotic resistance in bacteria.Thus, this study aimed to compare the antibacterial properties of honey from Apis cerana (Giant Honey bee) and Apis dorsata (Asian Honey bee) against Staphylococcus aureus and Escherichia coli. A trueexperimental research using the Kirby-Bauer Disk Diffusion Method was conducted to gather and analyze imperative data from the samples.This was conducted by the researchers at the De La Salle Medical and Health Sciences Institute's Angelo King Medical Research Center. Despite expectations, the results showed that honey from Apis dorsata and Apis cerana did not inhibit the growth of Staphylococcus aureus and Escherichia coli with 0 mm Zone of Inhibition. While the positive controls, Vancomycin and Ampicillin, showed notable inhibitory effects. The inability of honey from Apis cerana and Apis dorsata to inhibit the growth of Staphylococcus aureus and Escherichia coli underscores their limited antibacterial efficacy. The results highlight the complexity of honey's antibacterial properties, the exploration of alternative bacteria or fungi for experimentation, and the importance of employing chemical analysis of the honey samples for further research to identify factors influencing honey's effectiveness against bacterial strains.

First Advisor

Myra Michelle M. Mojica

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