Date of Completion

2024

Document Type

Research Project

Degree Name

Grade 12

Keywords

tetracycline, ELISA, Nile tilapia, Oreochromis niloticus, Taal Lake

Abstract

Tetracyclines are a group of antibiotics, commonly administered to fish to treat and prevent various bacterial infections. However, concerns have been raised regarding the development of antibiotic resistance in both fish and human populations. With the overuse of Tetracyclines in managing diseases in aquaculture comes the accumulation of antibiotic residues in the meat of the fish, which is consumed by the human population. Our study chose the Nile Tilapia (Oreochromis niloticus) as the target for quantification, as it is a species of fish that is widely cultivated and consumed in the Philippines. This study utilized a competitive Enzyme-Linked Immunosorbent Assay (ELISA) in quantifying the residue levels for Tetracyclines in the meat samples of sixty (60) adult Nile Tilapia from a farm cage in Taal Lake. The Maximum Residue Level of 200 µg/kg set by the Philippine National Standards (PNS) and the Bureau of Agriculture and Fisheries Standards (BAFS) was used as a point of comparison for the residue levels. Results from the ELISA testing determined an average residue level of 563.84 μg/kg among the 60 adult Nile Tilapia samples, and of the 60 adult Nile Tilapia samples, only 10 were found not exceeding the MRL. Then a one-way sample T-test was used to determine if there is a significant difference in the Tetracycline residue levels from the farm-caged adult Nile tilapia in Taal Lake and from the set MRL of 200 µg/kg. From the T-test, it was found that there is a significant difference between the mean of the sample residue and the MRL.

First Advisor

Ramgie M. Bartolata

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