Date of Completion

2024

Document Type

Research Project

Degree Name

Grade 12

Keywords

street food, antimicrobial susceptibility, microorganisms

Abstract

Street-vended foods which are widely accessible to Filipino consumers, prompt concerns regarding microbial safety due to the lack of regulation in the sector. This investigation specifically targets the identification of prevalent microorganisms in commonly sold street foods along Congressional Road, Dasmariñas, Cavite. Employing a descriptive research design, the study aims to detect microorganisms in commonly consumed street foods like kwek-kwek, kikiam, and turon. The methodologies include stomacher homogenization, serial dilution, plate spreading, plate streaking, VITEK analysis, and Kirby-Bauer disk diffusion to evaluate the presence of microorganisms and its antimicrobial susceptibility. The results have revealed the absence of microorganisms in the food samples of kwek-kwek and kikiam, whereas colonies of Bacillus cereus have been identified in the samples of turon. Bacillus cereus has maintained its susceptibility for vancomycin with an inhibition zone of 22 mm. It developed a resistance with ciprofloxacin with a zone diameter of 20 mm. B. cereus maintained its resistance to oxacillin with no zone of inhibition. These findings underscore potential threat of consuming turon due to the contamination of B. cereus. The developing antimicrobial resistance of B. cereus to ciprofloxacin also poses a threat to effective treatment and medication. Monitoring the food handling practices of the street food sector is crucial to prevent food contamination and the development of antimicrobial-resistant microorganisms.

First Advisor

Pauline L. Cueno

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