In vitro study of the antibacterial activity of 5 plant extracts (Hibiscus rosa-sinensis, Angelica keiskei koidzumi, Coleus aromaticus Benth, Cymbopogon citratus and Psidium guajava) against Escherochia coli and Staphylococcus aureus

Date of Completion

2018

Document Type

Capstone Project

Degree Name

Grade 12

Keywords

Plant Extracts, Antibacterial Agents, Echerichia coli, Staphylococcus aureus

Abstract

This study used an experimental research design. The study identified which of the five ethanolic crude extracts portrayed the best medication against E. coli and S. aureus. The significant difference between 100% concentration, 75% cocentration and 50% concentration of each plant extracts was calculated. The experiment was made possible by the use of Kirby-Bauer method or Disk Diffusion Assay. The results showed that Staphylococcus aureus is most susceptible to the ethanolic extract Psidium guajava (guava). As interpreted, there is a significant difference between the treatment groups with concentrations 100%, 75% and 50% of the said extract, determined through ANOVA. The researchers recommend soaking the plant parts in ethanol for 48 hours or more instead of the usual 24 to get a more potent extract, expound the antibacterial study of the said plant, perform the research with a synergistic approach and lastly, conducting a more extensive study in relation to the minimum inhibitory concentration through phytochemical analysis.

Comments

Call Number: RP SHS 18 45

Location: Research Commons

First Advisor

Pancho E. Ercilla

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