Optimization of a high-performance liquid chromatography method for quantification of cyclophosphamide residues in hospital-associated waters of a tertiary, private hospital in Cavite

Date of Completion

5-2019

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

Cyclophosphamide, Chromatography, High Performance Liquid Chromatography

Abstract

To quantitatively determine the presence of cyclophosphamide in the hospital-associated waters, a standard addition method using high performance liquid chromatography (HPLC) was developed and optimized. Effluent samples were collected at the stage 3 treatment of the sewage treatment platn in a tertiary, private hospital in Dasmarinas City, Cavite. The results showed that the drug could be effectively quantified in hospital-associated waters using a simple and widely accessible technology. However, there is currently lacking data for established minimum level of toxicity for cyclophosphamide to determine if this level already poses considerable risks when found in the aquatic environment. Further analysis of this level of cyclophosphamide in treated water systems using suitable animal models may prove to be beneficial in establishing possible toxic effects in humans.

First Advisor

Alicia P. Catabay, RPh, MSc, PhD

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