Evaluation of the hepatoprotective potential of methanolic extract of Caulerpa lentillifera against acetaminophen-induced liver toxicity in juvenile zebrafish (Danio rerio)

Date of Completion

5-2019

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

hepatoprotective, Caulerpa lentillifera, liver toxicity, zebrafish

Abstract

Juvenile zebrafish were exposed to μM concentrations of acetaminophen t induce liver damage. C. lentillifera methanolic extracts, were concomitantly added to individual tanks containing 10μM or 25μM APAP. Similar experiments were conducted with N-acetylcystiene/NAC and silymarin replacing C. Lentillifera extracts. Hematoxylin and Eosin staining revealed the extent of liver injury through the presence of hepatic necrosis, leukocyte infiltration, hepatocyte vacuolization and ballooning. The antioxidant mechanism of hepatoprotective activity was assessed by DPPH free radical scavenging assay. The study concluded that, 1) The histophatological analysis of the liver showed that C. lentillifera extract prevented the progression of liver damage caused by APAP. The results of DPPH free radical scavenging assay indicated that the hepatoprotective activity of C. lentillifera extract might have other antioxidant mechanisms aside from free radical scavenging. In order to effectively assess the improvement in the survival rate of juvenile zebrafish, longer exposure in the treatments is recommended.

First Advisor

Sigredo B. Mata, RPh

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