Determination of alpha-amylases inhibitors from selected Philippines plants using weak affinity chromatography

Date of Completion

2021

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

plant extracts, chromatography

Abstract

The research employs an experimental design with a quantitative approach. Amylase was immobilized in porous silica beads using a Schiff-based reduction method with sodium cyanoborohydride. The affinity column contained 190.83 nmol of amylase or 93.33 mg/g (amylae/silica). The silica-containing immobilized amylase was packed using Perkin Elmer High Pressure Liquid Pump at 200-250 bars. The complex mixtures used in this experiment were coconut water, Cocos nucifera (I.), and rambutan fruit, Nephelium lappaceum (L.)/ These two samples were tested in the unprocessed form while only the rambutan fruit was fractionated. The fractionation employed liquid-liquid fractionation using water and ethyl acetate. HPLC was used to analyze the affinity of sample extracts with amylase. The chromatograms of rambutan fruit extracts and coconut water extracts indicate that these extracts contain compounds that can bind to amylase. The reported Kd of the samples represents the binding affinity of amylase to the target molecules. The peaks observed in the chromatograms show that WAC may be used in the preliminary analysis of possible sources of amylase inhibitors.

First Advisor

Timothy Jay L. Bengala

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