Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

α-amylase, liquid-liquid partitioning, aqueous crude extract, G. oppositifolius (L.)

Abstract

Type 2 Diabetes mellitus is a chronic disorder profoundly influenced by daily variations in lifestyle determinants such as diet, exercise, infection, and stress which demands continuous management. It is a major health problem that has a high rate of morbidity and mortality which results in substantial healthcare costs. Glinus oppositifolius (Linn.), also referred to as “papait” in the Philippines, is a local vegetable weed commonly consumed in the Ilocos region and some parts of Luzon areas, believed to possess various medicinal properties, including its antidiabetic effects. This study evaluates the α-amylase inhibitory potential of the aqueous crude extract of the aerial parts of G. oppositifolius and its fractions (dichloromethane, butanol, and aqueous). Analysis of the plant’s phytochemical profile revealed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, tannins, and reducing sugars in the crude extract. The DCM fraction contained alkaloids, flavonoids, and glycosides; the butanol fraction contained flavonoids, glycosides, saponins, and trace reducing sugars; and the aqueous fraction contained all except alkaloids. TLC profiling using gugo as a standard for saponins indicated similar Rf values between the butanol fraction and gugo extract, suggesting the presence of saponins and triterpenoids. The α-amylase inhibitory effect was assessed using the 3, 5-dinitrosalicylic acid (DNS) assay, with the crude extract and its fractions compared to negative and positive controls (quercetin and acarbose). Results showed the highest inhibition in the DCM fraction, followed by the crude extract. The DCM fraction's absorbance compared to positive controls might indicate a higher inhibitory effect or less interference from reducing sugars. These results highlight the potential of G. oppositifolius, especially its DCM fraction, in inhibiting α-amylase activity. Furthermore, the aqueous crude aerial parts extract of G. oppositifolius shows promise for dietary purposes in managing carbohydrate digestion, warranting further investigation into its bioactive compounds and mechanisms.

First Advisor

Ryan Joseph C. Tuzon, RPh

Second Advisor

Renz Angelo G. Bunquin, RPh

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