Date of Completion

2025

Document Type

Research Project

Degree Name

Grade 12

Keywords

Bacterial contamination, public restrooms, toilet seats, colony-forming units (CFU)

Abstract

Toilet seats in public restrooms can harbor various microorganisms, posing potential health risks to frequent users. This study aimed to provide a descriptive analysis of bacterial diversity and contamination levels on toilet seats in men’s and women’s restrooms at Promenade Des Dasmariñas Park, Cavite. A descriptive research design was employed, with six samples collected during peak hours before cleaning operations. Samples underwent serial dilution, inoculation on Müller- Hinton agar plates, and incubation at 37°C for 24 hours. Colony-forming units (CFU) were counted, while selected isolates were subcultured, Gram-stained, and identified using the VITEK system. Results revealed that both men’s and women’s restrooms exhibited bacterial contamination, with CFU counts ranging from light to heavy levels. Bacillus spp. was identified in the women’s restroom, while Gram- positive isolates from the men’s restroom remained unidentified by the VITEK system. These findings emphasized the presence of microbial contaminants in public restrooms, highlighting the need for consistent cleaning and sanitation practices in high-traffic public parks. Routine monitoring of bacterial contamination could help ensure safer public facilities.

First Advisor

Pauline L. Cueno, LPT, MST

Download

Share

COinS