Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Aedes aegypti larvae, Bacillus thuringiensis israelensis, Biolarvicidal Activity, Diplazium esculentum (Edible fern) leaf extract, Synergism

Abstract

Heavy rainstorms were common natural disasters that occurred yearly in the Philippines. Such problems led to the formation of stagnant water, a breeding ground for vectors such as Aedes aegypti. Vector-transmitted diseases, most notably dengue, have been one of the major health concerns in the Philippines, affecting thousands of individuals yearly and taking lives when left untreated. This study evaluated the synergism between Bacillus thuringiensis israelensis and Diplazium esculentum against Aedes aegypti larvae. The study used experimental research and arithmetic means to analyze the efficacy of Bti and DEL alone and in combination. Kruskal-Wallis followed by post hoc test, Dunn’s method was also used to determine the significant difference of its combined mortality rate compared to the control groups. The Bti isolate was proven to have a lethal effect on the larvae, achieving an 86.6% mortality rate within 24Hr. Moreover, the varying concentration of DEL extract (20%, 40%, 60%, 80%) proves to have an inverse relationship with the duration of exposure. Furthermore, the combined effect of Bti and DEL proves to be more lethal compared to the positive control, in which it outweighs the mortality rate obtained by the control in every 4Hr period. Duration of exposure was also significantly reduced, achieving a 100% mortality rate within 20 hours, in contrast to the positive control that obtained 100% mortality after the 24Hr period. Using Kruskal-Wallis, a p-value of less than 0.05 was obtained in every 4Hr period, concluding a significant difference in larval mortality rate between the combined Bti+DEL and the control groups.

First Advisor

Pamfilo C. Bias, RMT

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