Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Dioscorea alata, hemagglutination, ammonium sulfate fractionation, hemagglutinating titer

Abstract

Species from the Dioscoreaceae family have been studied for their clinical implications, including blood typing. Studies have indicated the presence of lectins in Dioscorea spp., including Dioscorea alata, which suggests potential for hemagglutination research. To obtain the semi-purified lectin extract, crude lectin was extracted using phosphate-buffered saline. Ammonium sulfate fractionation was utilized for the semi-purification of the crude lectin extract, and different dialysate concentrations were prepared. Total protein content determination was performed for each dialysate to confirm the presence of protein. Only A and B blood groups were included. Selected participants underwent proper ethical considerations for their participation. To determine the hemagglutination activity of the lectins, 96-well U-shaped microtiter plates were utilized. Serial dilutions were performed up to the 24th well for each dialysate concentration to determine the hemagglutinating titer. Red cell suspensions were also prepared from the samples collected from each participant. The lectin dialysates of each concentration were able to exhibit hemagglutinating activities in an increasing trend with the 90% dialysate achieving the highest hemagglutinating titers for blood groups A and B.

First Advisor

Jhon Phillip S. Bautista, RMT, MSCLS

Download

Share

COinS