Date of Completion
2023
Document Type
Thesis
Degree Name
Bachelor of Science in Medical Laboratory Science
Keywords
Antimicrobial property, Goat Weed, Broth Microdilution, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration
Abstract
Ageratum conyzoides Linn, included in the family Asteaceae is commonly known as goat weed and damong pailaya. It is a tropical plant used as a treatment for diarrhea, scratches, antiinflammatory, snakebite, insecticide, and nematicide. On the other hand, Methicillin-resistant Staphylococcus aureus (MRSA) has been a major cause of disease and fatality since it first occurred in the 1960s, transmitted around the world, and is now one of the most common bacterial infections. The antimicrobial activity of the plant was evaluated against MRSA ATCC 43300 through the 2-fold dilution of the plant extract broth diffusion method to get the Minimum Inhibitory Concentration (MIC) of the A. conyzoides Linn and vancomycin. Then, Minimum Bactericidal Concentration was conducted upon determining the MIC. The phytochemical test for A. conyzoides Linn yielded a positive result for Flavonoids, Saponins, and Tannins. On the other hand, it yielded a negative result for Alkaloids. Using the broth microdilution method, A. conyzoides Linn showed more than 2 ug/ml growth or no inhibitory activity against MRSA and growth of more than 300 CFU/mL. Thus, A. conyzoides Linn had no bacteriostatic and bactericidal against MRSA.
First Advisor
Carolina B. Capili
APA Citation
Caldejon, A. B., Calero, N. B., Damiar, K. B., Daquigan, A. S., Del Rosario, J. G., Dimaano, P. C., & Edrad, A. L. (2023). In vitro antimicrobial leaf susceptibility testing of Ageratum conyzoides Linn (Damong Palaiya) against metchillin-resistant Staphylococcus aureus (MRSA). [Bachelor's thesis, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/bsmls/177