Date of Completion
2023
Document Type
Thesis
Degree Name
Bachelor of Science in Medical Laboratory Science
Keywords
Anti-biofilm Activity, Enterococcus faecalis, Carica papaya L. leaf extract, Minimum Inhibitory Concentration (MIC), Minimum Biofilm Inhibitory Concentration (MBIC), Minimum Biofilm Eradication Concentration (MBEC)
Abstract
The research aimed to determine the anti-biofilm properties of Carica papaya L. leaf extract against Enterococcus faecalis. The study used an experimental design to assess the anti-biofilm properties of Carica papaya L. leaf extract against Enterococcus faecalis. Biofilms formed by Enterococcus faecalis were subjected to crystal violet assay. Minimum Inhibitory Concentration (MIC) and Minimum Biofilm Inhibitory Concentration (MBIC) were determined to assess if the papaya leaf extract was able to inhibit the biofilm formed by Enterococcus faecalis after 24 hours of incubation. Minimum Biofilm Eradication Concentration (MBEC) was also determined to assess if the extract was able to eradicate the biofilm formed by Enterococcus faecalis after 48 hours of incubation. MIC and MBIC were incubated 24 hours before reading. The MIC, MBIC, and MBEC were found to be at 0.391%, 6.25%, and 25% concentrations of Carica papaya L. leaf extract respectively. The Carica papaya L. leaf extract has a potential anti-biofilm activity against Enterococcus faecalis.
First Advisor
Arianne Rose A. Lim
APA Citation
Lone, A. L., Lopez, C. Y., Maggay, Q. P., Mendoza, C. C., & Monterde, N. S. (2023). In vitro anti-biofilm activity of Carica papaya L. (Papaya) leaf extract against Enterococcus faecalis. [Bachelor's thesis, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/bsmls/156