Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Biotechnology

Keywords

Pomacea canaliculata, antimicrobial, Plate Count Agar, Macrogen 16S rRNA gene sequencing

Abstract

Pomacea canaliculata, also known as Golden Apple Snail, was recognized for their antibacterial and antioxidant properties, and was previously utilized as a delicacy. However, due to their destructive nature they eventually became a pest in rice farms. A wooden chopping board is a common tool used in public markets to slice meat, poultry, vegetables and other goods. However, due to its exposure and usage, the surface area of these chopping boards became an ideal breeding ground for all kinds of bacteria. While public markets continue to be popular for their cheaper prices, rising cases of diseases caused by bacteria growing on wooden chopping boards and improper food handling has led the researchers to explore solutions to natural antimicrobial resources and can benefit the well-being of the people. The study aimed to assess the inhibition of Pomacea canaliculata mucus on bacteria found on a wooden chopping board. Samples were swabbed from a wooden chopping board and streaked on Plate Count Agar for bacterial growth. After isolating a pure culture, collection of the snail species and their mucus was done. The Kirby Bauer test was conducted following the previous steps. After which, samples were sent to Macrogen South Korea for 16S rRNA gene sequencing, processing, alignment, and construction of a phylogenetic tree followed. Overall, the study provides an understanding of P. canaliculata as a prospective antimicrobial agent against bacteria found in wooden chopping boards, its antimicrobial activity and how it can prompt researchers and farmers to experiment with its antimicrobial characteristics, and the proper authorities to implement proper sanitation protocols when selling raw produce in public wet markets and lastly, to educate the communities on the importance of food safety and quality when purchasing goods.

First Advisor

April O. Bermejo-Ambita

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