Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Biotechnology

Keywords

Meta-analysis, pig microbiota, 16S rRNA gene sequences, Alpha diversity, Beta diversity.

Abstract

The domestic pig (Sus scrofa domesticus), sometimes known as the "pugok" or "native pig," is a widely distributed animal with unique genetic characteristics influenced by a variety of environmental factors and selected elements. This study used meta-analysis on the following variables: age, antibiotic exposure, diet, climate, and geographic location in order to validate changes in the compositions of the fecal microbiota in native pigs (Sus scrofa domesticus).The objective of this study was to comprehend the global differences in the fecal microbiota makeup of native pigs (Sus scrofa domesticus). Using 16S rRNA gene sequencing data from databases, a meta-analysis was conducted. High-throughput sequencing of the 16S rRNA gene, processed using the DADA2 pipeline for quality control and taxonomy identification, was one of the contemporary techniques used in the study. The findings revealed certain patterns in the composition of pig’s microbiome with respect to the age, antibiotic exposure, diet, climate, and geographic location. Particularly, comparable diets and agricultural techniques in North America and Europe resulted in comparable microbial assemblages, but tropical regions, such as those seen in Africa, fostered a diversity of microbial populations because of their distinct climatic circumstances and use of organic farming techniques. The study also validated the impact of age, observing that younger pigs had less diversified microbial communities than older pigs.

First Advisor

Nicole B. Marin

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