Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Biotechnology

Keywords

biochemical test, bioremediation, biogas, methanogen, polluted water

Abstract

Pollution continues to cause widespread damage to our environment; bioremediation can help the environment recover by utilizing the use of microorganisms to break down pollutants. This study aimed to isolate and identify methanogen from biogas production of Sus scrofa domesticus manure and to be used in the bioremediation of polluted water. The slurry composed of pig manure and water was placed inside of the digester, it has undergone four stages of anaerobic digestion. The first step is hydrolysis which is an initiation or conversion of complex molecules to soluble organic matter. Next is acidogenesis which is the conversion of simple sugar into carbon dioxide, hydrogen, ammonia, and organic acids. The third step is acetogenesis when acetogenic bacteria convert the organic acid into acetic acid, and lastly is methanogenesis where the methanogen bacteria convert intermediate products into biogas. The presence of methane (biogas) was confirmed and indicated the presence of methanogens. The methanogenic archaea was isolated from the biogas production of pig manure, it has undergone serial dilution, incubation, and cultivation in anaerobic chamber since it is strictly anaerobe. The identification of the organism was confirmed with the use of gram staining, biochemical testing, and morphological characterization. Polluted water samples were collected from the Imus River - Dasmariñas Station and treated with methanogenic isolates. Treated and untreated water samples were sent to a laboratory and subjected to various water parameter tests, resulting in an increase in temperature, turbidity, TSS, and conductivity; no difference in color, odor, pH, and chlorine residual; and a decrease in nitrate concentration from 7.03 mg/L to 2.90 mg/L which is a significant result since excessive amounts of nitrates can be toxic to humans and animals. Further research is needed to optimize the process and address the increase in other parameters to ensure overall environmental health improvement.

First Advisor

Iluminada A. Ronio

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