Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Biotechnology

Keywords

Arbuscular Mycorrhizae, Glomus sp., Biofertilizer, Allium fistulosum (spring onion)

Abstract

Sustainable agriculture is focused on optimizing available resources to ensure food security for present and future generations. Arbuscular Mycorrhizal Fungi (AMF) are important natural fertility-promoting microbes in the soils, enabling soil fertility and soil health by enhancing the performance of microbial communities, leading to increased growth rate and productivity. This study aimed to develop a biofertilizer using AMF for agricultural sustainability of Allium fistulosum (spring onions) under specific conditions. Physicochemical parameters such as soil bulk density, temperature, salinity, and pH were measured. Wet sieving method was then used to isolate and identify the presence of AMF in soil. T0 as control and T1 as experimental groups were observed over 4 weeks. A two-way Repeated Measures ANOVA was utilized to evaluate the impact of different treatments, unraveling the significant influence of AMF on the growth of the Allium fistulosum. Results showed that soil physicochemical parameters were within the optimal growth range, with values of 0.015 cm3 (soil bulk density), 25.67°C (temperature), 0.085 dS/m (salinity), and 7.05 (pH). Additionally, the presence of Glomus sp. AMF was identified in soil. ANOVA test results showed that, considering the accepted values (sig. level=.05, p-value=0.000), all values for both T0 and T1 were within the accepted p-value for the height variable; only T1 showed significant results for the width variable; and the majority in T1 showed significant results for the stem variable. Meanwhile, T-test results showed higher means in T1 for both the width and stem variables. These findings were essential in achieving the study’s objectives of contributing to agricultural sustainability.

First Advisor

Llyrha Mae M. Capio

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