Phytochemical analysis and determination of the in Ovo cytotoxic effects of Phyllanthus niruri Linn. leaf extracts against Salmonella enteritidis

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Cytotoxins.

Abstract

Phyllanthus niruri Linn, locally known in the Philippines as sammsampalukan, is traditionally used as a herbal remedy for treating cough, fever, viral infections, bacterial infections, and many other illnesses. In this study, its methanolic leaf extracts were subjected into an egg-based assay, evaluating its cytotoxic effects against Salmonella enteritidis. The methanolic leaf extract of P. ninuri L. was found to contain phytochemicals such as: flavonoids, tannins, alkaloids, saponins, terpenoids and steroids in considerable amounts and cardiac glycosides in a lesser amount. These compounds are attributed for the therapeutic effects of the methanolic leaf extract. Moreover, the bactericidal effects of the extract were observed to be efficient at concentrations of 1 to 10 ug/mL as no mortality within these concentrations were absorved, however, embryonic death was observed at 50ug/mL of the extract mixed with bacterial suspension within 48 h of post-inoculation.

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