In vitro screening of antimicrobial activity of selected Philippine ficus species
Date of Completion
2018
Document Type
Thesis
Degree Name
Bachelor of Science in Biochemistry
Keywords
Antimicrobial Agents.
Abstract
The three (3) Ficus species; F. minahassae, F. nota and F. pseudopalma, were screened for their secondary metabolites and tested for their antimicrobial activities using Kirby-Bauer well diffusion assay and determination of minimum inhibitory concentrations. Three concentrations; 10, 50 and 100 mg.mL, were used to determine the zone of inhibitiion of the hexane, dichloromethane and methanol extracts of the three Ficus species. At 100 mg/mL, the methanol and dichloromethane and methanol extracts of the three Ficus species. At 100 mg/mL, the methanol and dichloromethane and methanol extracts of F. nota exhibited inhibitory activities S. aureus, B. subtilis, E. coli, S. typhi and C. albicansa. At the same concentration, the methanol and DCM extracts of F. pseudopalma showed antibacterial activity against B. subtilis and S. aureus. The minimum inhibitory concentrations of the methanol and dichloromethane extracts of F. nota were 12.5 and 50 mg/mL respectively and for the dichloromethane and methanol extracts of F. pseudopalma, the concentrations were: 12.5 and 50 mg/mL for B. subtilis and S. aureus respectively. To best of our knowledge, this is the first report of the antifungal activities of F. nota, and the antibiotic activities of the three plant samples against S. typhi, and the antimicrobial activities of F. minahassae which did not exhibit inhibitory activities against the selected microbes at 100 mg/mL concentration.
APA Citation
Anteja, N. T., Lopez, R. V., & Nolasco, A. D. (2018). In vitro screening of antimicrobial activity of selected Philippine ficus species. [Bachelor's thesis, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/bch/43/