Date of Completion


Document Type


Degree Name

Bachelor of Science in Biochemistry


Biosynthesis, antibacterial activity, zinc oxide, nanoparticles, Croton Tiglium, Croton, leaf extract


In the present study, the biosynthesis of zinc oxide nanoparticles was achieved by using the aqueous leaf extract of Croton tiglium. The biosynthesized zinc oxide nanoparticles were characterized using various techniques such as UV_Vis spectrophotometer (UV-VIS), Energy Disperse X-ray (EDX) analysis and scanning electron microscopy (SEM). The optimum incubation time and temperature was determined and found to be at one hour and 80 ‎°C respectively for the synthesis of zinc oxide nanoparticles. The maximum wavelength of absorption occurd at 364 nm which is agreemnt with that was referred in citation. The morphology of synthesized nanoparticles was determined using SEM analysis that showed rod like structure. On the other hand, the chemical composition of the zinc oxide nanoparticles were analyzed using EDX wherein analysis revealed a 77.04% of zinc and 22.96% of oxygen nanoparticle composition. To determine the antibacterial activity of synthesized ZnO nanoparticles. Escherichia coli and Staphylococcus aureus was used as a representative for gram.

First Advisor

Eduardo B. Tibayan Jr., MSc, PhD