Date of Completion

7-2019

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Mimosa, Plant Extracts, Anti-Bacterial Agents

Abstract

Proteins that have the ability to inhibit the growth of microorganisms are called antimicrobial proteins. This study was conducted to extract and partially characterize proteins from the Mimosa pudica Linn stem and leaf combined extract. The proteins were extracted using 0.1M phosphate buffer via grinding, filtering and centrifugation. The presence of proteins was confirmed using chemical tests such s the Biuret, Ninhydrin and Xanthoproteic test. The sample was subjected to Bradford assay, to detect the crude protein concentration using different dilutions which were 1:2; 1:5 and 1:10. The concentration of the crude protein obtained was 1.780 mg/mL. The percent yield was determined to be 0.1086%. Using SDS PAGE, the presence of protein subunits were confirmed in the sample. The extracted proteins did show antibacterical activity against Escherichia coli and Staphylococcus aureus when subjected to Kirby-Bauer disc diffusion method. Significant difference in the zone of inhibitions was observed as the concentration of the crude protein increased. REsults for zone inhibition confirm that the protein isolates had antibacterial properties.

First Advisor

Iluminada A. Ronio

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