Title

Antimicrobial properties of bioluminescent bacteria from Decapterus macarellus

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Anti-Infective Agents.

Abstract

Antibiotic resistance is one of the major public health problems because of reduced effectiveness of drugs which makes the antibiotic-resistant bacteria difficult to be killed. This study aims to search for novel sources of antibiotics. In the field of bioluminescence, there is a potential new bioresource of antibiotics which is bioluminiscent bacteria. Bioluminescent bacteria are motile, gram-negative, non-spore forming and found in 80% marine organisms. In this study, it was isolated from the epedermis, adipose eyelid, stomach mucosa and intestine of the Decapterus macarellus (mackeral scad or galunggong) and tested against Klebsiella pneumonia, Staphyloccocus aureus and Candida albicans. Morphological and cultural characteristics were determined. Biochemical test were also conducted whereas catalase and MR-positive results and VP, indole and citrate-negative results were obtained while in TSI test, most had alkalike slant and acidic butt. Optimum condition of the bioluminescent bacteria using agar and broth were determined which was at 22°C with 25% and 50% NaCl for agar while 4°C with 75%NaCl for broth. The antimicrobial activity of bioluminescent bacteria against Klebsiella pneumoniae and Staphyloccus aureus were present but absent in Candida albicans.

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