Date of Completion

7-2019

Document Type

Research Project

Degree Name

Grade 12

Keywords

angiogenesis, Angiogenesis Inducing Agents, chorioallantoic membrane, ethanolic extract, Eleusine

Abstract

Eleusine indica is a weed commonly known as paragis in the Philippines and mostly found on tropical countries. The purpose of the study is to identify the angiogenic effect among the varying concentrations of E. indica extract on the Chorioallantoic membrane of a ten-day old duck embryo via angiogenesis which was known as the formation of new blood vessels originating from the pre-existing blood vessels. Three different concentrations: (100ppm), (200ppm), (300ppm), and a (T0) using saline solution were used in 20 duck eggs to identify its angiogenic effect. Upon extraction, the leaves were soaked in 99% ethyl alcohol and a rotary evaporator was used to get the 100% leaf ethanolic extract, diluted and administered to the eggs using pipettes. Then, the eggs were incubated for 48 hours and then counted the vessels. The gathered result had an average of (T0) 8.6, (T1) 9.8, (T2) 6.4 and (T3) 9.8. Based on Analysis of Variance, the three treatments had almost the same number of collaterals counted with a close average in comparison to (T0). The study failed to reject the null hypothesis. Therefore, a no significant difference on the effects of the concentrations of E. indica leaf ethanolic extract on the Chorioallantoic membrane of a ten-day old duck embryo had been concluded.

First Advisor

Pauline L. Cueno

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