Date of Completion

2025

Document Type

Research Project

Degree Name

Grade 12

Keywords

microbial contamination, street food vendors, Indian mango, water contamination, VITEK® analysis, Salmonella spp.

Abstract

Street food vendors, specifically Indian mango (Mangifera indica) stalls are informal businesses that offer food and beverages to the public within communities. Despite its popularity and prevalence in the Philippine context, there is minimal localized data and monitoring on potential microbiological contamination in food preparation processes and resources. To address this gap, 50mL water samples were randomly collected from SFVs within Burol, Dasmarińas, Cavite. The samples underwent the serial dilution method, pour plate method, and streaking ,ethod on Mueller-Hinton Agar (MHA) plates for preparations for the incubation of potential microorganisms in the sample. After a 24-hour incubation at 37°C, asubculture exceeding 300 CFU/mL underwent VITEK® 2 Plus identification and Antimicrobial Susceptibility Testing. The findings revealed that Salmonella spp. contaminated the water sample, with its Antimicrobial Susceptibility Profile revealing susceptibility to meropenem, imipenem, ciprofloxacin, sulfamethoxazole, ampicillin, and ceftriaxone with a Minimal Inhibitory Concentration (MIC) spanning from 0.25- 2.00. Moreover, all MHA plates exceeded the Maximum Allowable Level of 300 CFU/mL that the Philippine National Standards for Drinking Water set by the Department of Health states. The findings emphasized widespread water contamination monitoring from Local Government Units for food-borne illness prevention.

First Advisor

Pauline L. Cueno, LPT, MST

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