Date of Completion

2025

Document Type

Research Project

Degree Name

Grade 12

Keywords

Public Markets, Vibrio Parahaemolyticus

Abstract

Aquaculture plays a crucial role in Southeast Asia, particularly in the Philippines, where shrimp farming is a significant industry contributing to food security and economic growth. This study aimed to detect the presence of presumptive Vibrio parahaemolyticus in the hepatopancreas of Litopenaeus vannamei (whiteleg shrimp) sourced from three major public markets in Dasmariñas, Cavite. A total of 105 shrimp samples were collected and analyzed using colony morphology on TCBS agar, identifying presumptive V. parahaemolyticus in 37 samples. The Central Market exhibited the highest prevalence (80%), followed by Luzviminda Market (14.3%) and Marketmall Public Market (11.4%). These findings highlight the varying levels of contamination, potentially influenced by differences in handling, storage, and overall sanitation practices among markets. The study emphasized the importance of implementing proper food safety measures, such as maintaining cold chain storage, improving hygienic handling, and regular disinfection of market facilities, to reduce bacterial contamination. Routine microbial monitoring was crucial for ensuring the safety and quality of shrimp sold to the public. Further research using molecular techniques is recommended to confirm these findings and provide a more accurate assessment of V. parahaemolyticus prevalence. Additionally, exploring innovative farming methods and stricter compliance with hygiene protocols in shrimp production and distribution can significantly enhance consumer protection and product quality.

First Advisor

Ramgie M. Bartolata, LPT

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