Date of Completion

2025

Document Type

Research Project

Degree Name

Grade 12

Keywords

Water Pollution, Bioluminescent Bacteria, Squid Ink

Abstract

Bioluminescent bacteria are light-emitting microorganisms that are commonly found among marine species. In this study, bioluminescent bacteria was isolated from squid samples and submerged in selected water samples from Ylang-Ylang River and Dasmariñas Zone I River in Dasmariñas, Cavite to determine whether it can be a potential water pollution indicator based on its bioluminescence intensity. The squid ink extracted from 6 squid samples was streaked on marine agar plates and incubated for 24 hours. The microplates were filled with 0.75 μL bioluminescent bacteria and 0.75 μL of polluted water samples and sterile water per three rows, where the light intensities were measured from 450 nm to 600 nm using a microplate reader. The results showed a significant relationship between the bioluminescence intensity and water pollution. Based on the findings, the Dasmariñas Zone I River had a mean of 0.090 (p< 0.05) compared to sterile water with 0.092 (p< 0.05), which exhibited a decrease in bioluminescence intensity as the water became more polluted. Meanwhile, the Ylang-Ylang River had a mean of 0.170 (p< 0.05) compared to sterile water with 0.134 (p< 0.05), which exhibited an increase in bioluminescence intensity as the water became less polluted. Therefore, the bioluminescent bacteria was a possible bioindicator of water pollution. Future research may conduct analysis on other water parameters to identify their significant relationship to the increase of the bioluminescent bacteria.

First Advisor

Pauline Cueno, LPT, MST

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