Date of Completion

2024

Document Type

Research Project

Degree Name

Grade 12

Keywords

Staphylococcus epidermis

Abstract

The healthcare industry is facing the challenge of antibiotic resistance. To find potential natural resources that can help solve this problem, this study was conducted to assess the antibacterial properties of different parts of Bambusa blumeana, namely the outer culm and shoots. The study also looked into the synergistic effect of these extracts against gram-positive and negative bacteria represented by Staphylococcus epidermidis and Escherichia coli, respectively. The Kirby Bauer Method, also known as the disc-diffusion method, was used to measure the Zone of Inhibition of the extracts or the ability of the extracts to inhibit the growth of the bacteria, S. epidermidis and E. coli. The data collection for this study was conducted at De La Salle Angelo King Research Center through Experimental Design. The results showed that the extracts of the outer culm and the shoots and its combined extracts showed 0mm of Zone of Inhibition against Staphylococcus epidermidis and Eschirichia coli. In comparison with the positive control, Gentamicin, that was used in this study, with a ZOI of 31.67mm on S. epidermidis and 35.00mm on E. coli, this study suggests that Bambusa blumeana ethanolic extracts do not show any inhibitory effects to these particular types of bacteria, namely S epidermidis and E coli. This study would be beneficial to the health field, specifically the pharmaceutical industry. Further investigation is recommended utilizing different methods of extraction and susceptibility tests against other bacterial strains for a more accurate result in determining antibacterial activity.

First Advisor

Vivian B. Ramirez

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