Date of Completion

2024

Document Type

Research Project

Degree Name

Grade 12

Keywords

Diospyros blancoi, Staphylococcus epidermidis, zone of inhibition, agar well diffusion, ethanol extraction, antibacterial activity

Abstract

Persimmon leaves (Diospyros blancoi) contain a variety of phenolic chemicals, including sterols, triterpenes, flavonoids, alkaloids, saponins, glycosides, and tannins. The study used an experimental research approach to evaluate the phytochemical properties and antibacterial activity of Diospyros blancoi ethanolic leaf extract against Staphylococcus epidermidis, one of the causes of nosocomial infections. D. blancoi leaves were submitted to the Department of Science and Technology (DOST) for phytochemical analysis and ethanol extraction. Agar well diffusion was used to test the antibacterial activity of D. blancoi extract against S. epidermidis. The phytochemicals show that D. blancoi leaves have health benefits like antioxidant, cholesterol- lowering, cardioprotective action, anti-inflammatory, antidiabetic, antifungal, insecticidal, and anti-feeding effects. According to the results, the concentrations of 20 mg/mL, 40 mg/mL, and pure extract yielded an average inhibition zones of 13.67 mm and 16.63 mm. Based on the results of the Independent T-test, 20 mg/mL, 40 mg/mL and pure extract have a p-value (0.000, 0.000, 0.001) that are less than 0.05 level of significance indicating that the concentrations have a significant difference when compared to cefoxitin. This suggests that S. epidermidis is resistant to the extract. Following these findings, application of different types of extraction, usage of higher concentrations, combination with antibiotics, and usage of different bacteria might be utilized to further investigate the antibacterial capabilities of D. blancoi leaves, moreover, identify and evaluate other natural plants that could be employed as antibacterial agent against S. epidermidis and other microorganisms.

First Advisor

Pauline L. Cueno

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