Date of Completion

2024

Document Type

Research Project

Degree Name

Grade 12

Keywords

Chorioallantoic membrane assay, Anas platyrhynchos, Citrus grandis, anti-angiogenesis

Abstract

Anti-angiogenesis has been proven to play a pivotal role in cancer and tumor management. Citrus grandis (Pomelo) rinds, rich in vital bioactive compounds, have shown potential anti-angiogenic properties. However, its efficacy in inhibiting angiogenesis in animal models remains unexplored. This study aimed to evaluate the anti-angiogenic activity of Citrus grandis rind ethanolic extract, specifically towards the number and length of blood vessels on the chorioallantoic membrane (CAM), to contribute to the development of alternative treatments for inhibiting tumor-associated angiogenesis and controlling cancer progression. The method of an in-vivo CAM assay was utilized, with Anas platyrhynchos (Mallard duck) embryos as test subjects, following three phases: pomelo rind extract isolation, administration of treatment and incubation, and observation of characteristics post-treatment. Statistical analysis revealed no consistent trend between the extract and angiogenesis, with variability in the duck embryo survival rate post-administration. This emphasizes the need for continued investigatory efforts to further discover the potential of natural products, especially against angiogenesis, towards improved cancer management. Such endeavors would require, overall, an improved methodological process to ensure data accuracy that would be more constructive toward cancer and health research.

First Advisor

Ramgie M. Bartolata

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