Date of Completion

2024

Document Type

Research Project

Degree Name

Grade 12

Keywords

Flammulina filiformis, Escherichia coli, Antibacterial Activity, Zone of Inhibition, Antibacterial Compounds, Kirby-Bauer Diffusion

Abstract

Mushrooms are commonly used in various industries for their healing properties, with an increasing number of studies affirming their potential as sources of novel antibacterial compounds. With the rising threat of antibioticresistant bacteria on a global scale, there has been an urgent need to find good alternative antibacterial treatments. This study aims to contribute towards combating this issue by providing an additional option for alternative medicine that is affordable, accessible, and sustainable. The study screened a commonly consumed mushroom in Asian cuisine, Enoki Mushrooms, for the presence of antibacterial activity against a common gram-negative bacterium—Escherichia coli. Researchers utilized quantitative experimental research design and conducted the experiment via the Kirby-Bauer Disc Diffusion method to determine a cause-and-effect relationship between the extracts from the Enoki Mushroom and the diameter of the zone of inhibition. The results show that different concentrations of Enoki Mushroom exhibited an acceptable mean zone of inhibition of greater than 15 millimeters (>15mm), which means that the Enoki Mushroom extracts tested positive for its antibacterial activity against E. coli. Given the positive results for antibacterial activity, researchers conclude that the Flammulina filiformis (Enoki Mushroom) has potential to be considered as an antibiotic agent against gram-negative bacteria.

First Advisor

Myra Michelle M. Mojica

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