Date of Completion

7-2019

Document Type

Research Project

Degree Name

Grade 12

Keywords

Allium Cepa, Antimitotic Agents, Onions, Diplazium Esculentum, Adiantum capillus-veneris

Abstract

Antimitotic agents are able to arrest cell division and are commonly used in cancer studies and treatment. The study aimed to compare the antimitotic activity of extracts Diplazium esculentum (Pako) and Adiantum capillus-veneris (Alambrillo), two ferns that contain the phytochemical flavanoid which could inhibit mitosis, on root tip mitosis of Onion. The ferns were separately dried, powdered, macerated in ethanol, undergone rotary evaporation and diluted in 200 ml distilled water. Onion roots were exposed to the fern extracts, tap water and Colchicine for 96 hours away from direct sunlight. After 96 hours, roots were cut and was fixated with Acetic Ethanol for 24 hours, then was stained with acetocarmine for 10 minutes and cells were viewed with a microscope. The data was gathered through manually counting meristematic cells and calculated with the mitotic index and was analyzed using anova and t-test. The results showed that Diplazium esculentum (Pako) had greater antimitotic activity with a lower mean mitotic index of (62.00) on onion root tips than Adiantum capillus-veneris (Alambrillo) with a higher mean mitotic index of (78.17), with a p-value of 0.011 indicating a significant difference between them, however, ANOVA from the mitotic index of the fern extracts and Colchicine showed a p-value lower than 0.05 which indicates that despite Diplazium esculentum (Pako) showed significantly greater antimitotic activity than Adiantum capillus-veneris (Alambrillo), it is still not as effective as Colchicine in inhibiting mitosis on the onion root tip.

First Advisor

Myra Michelle M. Mojica

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