Date of Completion

2023

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

Aluminum Chloride test, antioxidant activity, DPPH assay, Folin-Ciocalteu, Ipomoea aquatica Forssk., thin layer chromatography, total phenolics and flavonoids content

Abstract

Ipomoea aquatica Forssk., a member of the Convolvulaceae family, is locally known as “kangkong” in the Philippines or Water spinach in other areas around the world. I. aquatica is primarily known to be a food item that is used in a variety of dishes, but due to the presence of flavonoids and phenols, it also shows high antioxidant activity. The study utilized the use of the leaves and stem of I. aquatica which were subjected to different drying methods, specifically air and oven-drying. The samples were macerated and subjected to Kupchan partitioning. Thin Layer Chromatography (TLC) was then done to confirm and identify phytochemical compounds present in each sample. The FolinCiocalteu test and the aluminum chloride test were done to quantify the amount of phenols and flavonoids present in the fractions, wherein the fractions with the highest values were subjected to the DPPH assay to determine their antioxidant activities. It was observed that the ethyl acetate extract of oven-dried stems was the fraction with the highest % radical scavenging activity. There was little to no difference between the stems and leaves in terms of % radical scavenging activity. The effect of temperature, on the other hand, had varying effects on the antioxidant activities.

First Advisor

Ryan Joseph C. Tuzon

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