Bioassay-guided fractionation and determination of antifungal activity of ripe and unripe peel of saba banana (Musa acuminata x balbisiana)

Date of Completion

2019

Document Type

Thesis

Degree Name

Bachelor of Science in Pharmacy

Keywords

Banana, Chromatography, Antifungal Agents

Abstract

Phytochemicals and antifungal activity of ripe and unripe saba banana peels were studied. Kupchan partitioning method was used to extract and fractionate phytochemicals from ripe and unripe saba banana peels. These were then identified using phytochemicals tests and thin layer chromatography. Kirby-Bauer Disk Diffusion was then performed to determine the zone of inhibition of the Kupchan fractions, positive, and negative control. The study was statistically analyzed using the One-Way ANOVA test. In this study, it was found out that ripe saba peels have carbohydrates, phytosterol, flavonoid, amino acids, and terpenoid while unripe saba peels only have phytosterols, flavonoids, and amino acid. Among the fractionated samples, only the ripe hexane, and both unripe and ripe ethyl acetate were observed for a zone inhibition against Candida albicans at 1200Ug/desc.

First Advisor

Romeo Luis F. Ramirez

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