Nephrotherapeutic activity of brassica oleracea var. italica (Brocolli) florets crude extract on Cisplatin-induced Nephrotoxicity in female Sprague Dawley rats

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Brocolli, Rats, Cisplatin

Abstract

This study used an experimental design with four control groups. Cisplatin was used to induce kidney damage at a single intra peritoneal dose of 6 mg/kg body weight. Renal injury is then confirmed after 5 days with kidney function tests. Different concentrations of B. oleracea var. italica extract (100 adn 200 mL/kg) was induced for 8 consecutive days. After induction of the B. oleracea var. italica crude extract, BUN, creatinine, hematocrit and hemoglobin were measured. Based on the results, the study concluded that the increased levels of hemoglobin and hematocrit indicate that the kidneys which are the site of erythropoietin production are well and able to provide oxygen to the body, thus eliciting therapeutic response to the kidneys. The optimal concentration of the brocolli extract which elicit a positive response is either 100 and 200 mg/kg because both are effective and able to treat the damage produced by the cisplatin. Histopatholigc examination of the glomeruli was also evaluated. Group1 correlated with histopathologic examination indicating ciplatin sever necrosis of the renal tubules in the cortico-medullary junction with minimal fibroblasts proliferation that indicates renal toxicity was seen. Groups 3 and 4 at the 3rd histopathologic examination exhibited muld to moderate necrosis of the renal tubules in the cortico-medullary junction with mild fibroblasts proliferation which correlates to the nephrotherapeutic activity of B. oleracea var. italica crude extract.

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