Title

Hemagglutination properties from the crude extract of the corms of Colocasia esculenta (Taro)

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Hemagglutination, Colocasia

Abstract

This study used experimental research design. The crude extract was obtained from pulverized corms of Colocasia esculenta (Taro), defatted with the use of N-Hexane and was homogenized with the use of phosphate buffered saline (PBS) with a pH of 7.2. Blood samples were collected from one hundred and eight patients with blood types A, B, AB and O. 5% red cell suspension of blood types A, B, AB and O were added to different concentrations of the extracted lectin (50 mL/mL, 75 mL/mL and 100 mL/mL). The macroscopic evaluation made use of the red cell antigen-antibody reaction serologic grading standard chart, while the microscopic evaluation was done with the use of a microscope under a high-powered objective. The results showed that majority of the hemagglutination gradings in all blood groups were trace to 1+. Lastly, majority of the hemagglutination gradings in all blood groups were trace to 1+. The study has established that the lectin of Colocasia esculenta is not specific and cannot be used as an alternative typing serum considering that the blood types A, B, AB and O all displayed agglutination. However, this may not be generalized since the researchers did not subject to purifying the crude extract which might be an important factor in producing a specific lectin.

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