Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Anthocyanin, Anti-angiogenesis, Chorioallantoic Membrane assay, Dioscorea alata L.

Abstract

This study evaluates the anti-angiogenic properties of the aqueous methanolic extract of Dioscorea alata L. tubers using the chorioallantoic membrane (CAM) assay. The main objective of the study was to determine the extract’s ability to inhibit angiogenesis, a significant process in tumor growth and related pathological conditions. Qualitative phytochemical analysis confirmed the presence of various bioactive compounds, with carbohydrates being the most abundant phytochemical constituent present in the extract. Meanwhile, the pH differential method revealed that the extract has a high anthocyanin concentration of 1549.66 ± 65.21 mg CGE/L. The CAM assay demonstrated a dose-dependent anti-angiogenic effect, with significant reductions in the number of blood vessel branching points observed with increasing extract concentrations when compared to controls. The pre-treatment mean branching points are the following: 25% (139.53), 50% (146.19), 75% (138.79), 100% (181.80), Blank (160.14), Distilled Water (187.81), and Quercetin (253.50). While, the post-treatment mean branching points are the following: 25% (120.41), 50% (70.13), 75% (47.64), 100% (58.40), Blank (195.14), Distilled Water (161.81), and Quercetin (62.71). Statistical analyses using paired T-test and ANOVA confirmed significant differences in anti-angiogenic activity between the treatments and controls. These findings suggest that Dioscorea alata L. methanolic tuber extract has potent anti-angiogenic properties, supporting its potential therapeutic application in diseases related to angiogenesis.

First Advisor

Joseph Israel R. Guanlao, RMT, MSMT, MPH

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