Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Oryza sativa (Rice) leaves, Klebsiella pneumoniae, antibiofilm activity, phytochemical screening, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), biofilm inhibition, natural antibacterial agents

Abstract

This study investigated the antibiofilm activity of crude Oryza sativa (Rice) leaves extract against Klebsiella pneumoniae ATCC 700603. The research aimed to identify the phytochemical components of the extract and determine its Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), Minimum Biofilm Inhibitory Concentration (MBIC), and Minimum Biofilm Eradication Concentration (MBEC). Phytochemical screening revealed the presence of saccharides, reducing sugars, anthraquinone glycosides, tannins and phenolic glycosides, triterpenoids, cardiac glycosides, saponins, flavonoids, and alkaloids, with saccharides and reducing sugars found in the highest abundance. The MIC of the crude extract was determined to be 0.19%, while the MBC was 0.39%, indicating both bacteriostatic and bactericidal effects. However, no measurable MBIC or MBEC values were observed, as none of the tested concentrations completely inhibited or eradicated biofilm formation, though partial reductions were noted. These results suggest that the crude rice leaf extract contains active compounds capable of inhibiting and killing planktonic Klebsiella pneumoniae cells but remains ineffective in eliminating mature biofilms. The findings highlight the potential of Oryza sativa leaves as a source of antibacterial agents, while also emphasizing the need for further purification and concentration of its bioactive components to enhance antibiofilm efficacy.

First Advisor

Maria Katrina B. Pilit, RMT, AHI(AMT)

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