Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Selenium Nanoparticles, Klebsiella pneumoniae, Streptococcus pyogenes, MIC, Green Synthesis

Abstract

The current rise in antibiotic-resistant bacteria highlights the urgent need for alternative antimicrobial strategies. Selenium nanoparticles (SeNPs) have gained attention due to their potent antimicrobial properties, biocompatibility, and lower toxicity compared to other metallic nanoparticles. Their ability to be topically applied or ingested, along with the possibility of green synthesis, makes them both effective and environmentally sustainable. In this study, green-synthesized SeNPs were characterized and confirmed through several techniques. UV-Visible spectroscopy revealed a peak absorbance at 345 nm, while Fourier Transform Infrared Spectroscopy (FT-IR) identified functional groups including O-H (3300–3400 cm⁻¹), C=O (1630–1650 cm⁻¹), C-N (1400 cm⁻¹), and C-O-C (1000–1100 cm⁻¹). Scanning Electron Microscopy (SEM) showed spherical SeNPs ranging from 26.0 to 71.1 nm in size. Antibacterial activity was tested against Streptococcus pyogenes and Klebsiella pneumoniae. The Minimum Inhibitory Concentration (MIC) for both bacteria was found to be 0.1953125 µg/mL. No statistical differences were observed between the SeNP concentrations (6.25 to 0.1953125 µg/mL) and the positive control, Levofloxacin, for S. pyogenes. Similarly, K. pneumoniae exhibited no statistical differences when compared to the control, though absorbance readings were inconsistent across concentrations, possibly due to nanoparticle aggregation and low yield. Despite these limitations, the results demonstrate the promising antibacterial potential of SeNPs, suggesting their viability as an alternative treatment for infections caused by antibiotic-resistant bacteria.

First Advisor

Jion P . Dimson, RMT , MSMLS

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