Date of Completion
2025
Document Type
Thesis
Degree Name
Bachelor of Science in Medical Laboratory Science
Keywords
Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, Polymerase Chain Reaction, mecA gene, Agarose Gel Electrophoresis, Mannitol Salt Agar
Abstract
Public transportation is a critical space for human interactions across diverse populations and socioeconomic backgrounds, making it a significant medium for transmitting pathogens such as MRSA. Methicillin-resistant Staphylococcus aureus (MRSA) is a significant public health concern globally. It is a major cause of nosocomial infections and is increasingly linked to community-acquired infections. By utilizing Conventional Polymerase Chain Reaction, the researchers explored the presence of the mecA gene, a gene unique in MRSA, in public utility vehicles, specifically tricycles, in Dasmarinas City, Cavite. The researchers included Culturing and Isolation, DNA Extraction, Gel Electrophoresis, and Conventional PCR in their methodology to detect the presence of the mecA gene. Based on the results, the researchers found that Staphylococcus aureus is present in tricycles located in Dasmarinas City, Cavite. In addition, the researchers noted the presence of the mecA gene, which indicates the presence of MRSA among the samples that tested positive for Staphylococcus aureus.
First Advisor
Christine Ann P. Aromin, RMT
APA Citation
Bautista, H. D.,
De Castro, S. I.,
Herrera, M. M.,
Mercado, F. P.,
&
Rodil, V. P.
(2025).
Conventional PCR-based molecular detection of the mecA gene in methicillin – resistant Staphylococcus aureus (MRSA) isolated from selected tricycles in the City of Dasmariñas Cavite, Philippines.
Bachelor of Science in Medical Laboratory Science.
Retrieved from https://greenprints.dlshsi.edu.ph/bsmls/243