Date of Completion
2025
Document Type
Thesis
Degree Name
Bachelor of Science in Medical Laboratory Science
Keywords
BlaGES, ESBL, Escherichia coli, surveillance, treated water
Abstract
Extended-Spectrum Beta-Lactamase-Producing Escherichia coli (ESBL-producing E. coli) and its ability to survive in wastewater poses a great concern to the environment. This study shows the primary surveillance of ESBL-producing E. coli in a tertiary hospital’s water treatment plant. Multiple-tube fermentation technique was used in the determination of MPN while real-time polymerase chain reaction was utilized to confirm the presence of the blaGES gene in the isolated E. coli. Samples were collected in fifteen replicates for each month. The data gathered showed that all monthly samples showed an MPN of >8.0/100mL. All samples also demonstrated a negative result in RTPCR for the blaGES gene, signifying that no blaGES-positive ESBL-producing E. coli was observed during the three-month surveillance period.
First Advisor
Jennilyn Nicole G. Mendoza, RMT
APA Citation
Abela, W. S.,
Bolivar, A. T.,
Dela Cruz, K. F.,
Lagera, S. P.,
Monteza, D. U.,
&
Sabalza, M. J.
(2025).
Primary surveillance of blages-positive extended-spectrum beta-lactamases-producing Escherichia coli (ESBL-E coli) in treated sewage water from a selected tertiary hospital in Dasmariñas, Cavite.
Bachelor of Science in Medical Laboratory Science.
Retrieved from https://greenprints.dlshsi.edu.ph/bsmls/247