Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Anti-angiogenesis, Aloe vera, Selenium Nanoparticles, Sodium Selenite, CAM Assay, Green Synthesis of Selenium Nanoparticles

Abstract

This study evaluated the anti-angiogenic effects of Aloe barbadensis Miller (Aloe vera) leaf extract (ALE), sodium selenite (SSE), and green-synthesized selenium nanoparticles (SeNPs) using the chick chorioallantoic membrane (CAM) assay. Treatments at 25%, 50%, 75%, and 100% concentrations were assessed for their ability to inhibit vascular branching. ALE showed the most potent, dose-dependent anti-angiogenic activity, with 100% ALE significantly outperforming controls. SSE also inhibited angiogenesis at all concentrations but with less consistent trends. SeNPs demonstrated significant inhibition only at 25%, with decreased efficacy at higher concentrations. This was attributed to nanoparticle agglomeration and instability, leading to biological interference and reduced activity. Statistical analysis showed no synergistic effect of SeNPs over their precursors. In conclusion, Aloe vera extract proved to be the most reliable anti-angiogenic agent. While SeNPs hold theoretical promise, their current formulation exhibited interference rather than enhancement, indicating a need for improved nanoparticle stability.

First Advisor

Roxanne D. Rodriguez, RMT, MSCLS

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