Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Stingless Bees, Antifungal

Abstract

In light of the escalating global concerns regarding antifungal resistance and the limited availability of effective treatments for fungal pathogens, this study rigorously examines the antifungal efficacy and phytochemical composition of propolis extract. Sourced from Tetragonula biroi, a highly abundant and native stingless bee species to the Philippines, this research systematically investigates how the rich array of bioactive secondary metabolites within its propolis contributes to its potential as a novel natural antifungal agent. The ethanolic propolis extract, obtained via rotary evaporation, underwent comprehensive qualitative and quantitative phytochemical analysis to identify and quantify its bioactive secondary metabolites, specifically flavanonoids, phenolic compounds, and terpenoids. Quantitative analysis revealed a high content of bioactive secondary metabolites, with phenolics being the most abundant (243.92 mg GAE/g), followed by flavonoids (22.91 mg QE/g) and terpenoids (8.50 LE/g), all known for their potent antimicrobial properties. The extract demonstrated significant antifungal activity against Aspergillus niger, with a minimum inhibitory concentration (MIC) of 250 mg/mL (90.21% inhibition) over 72 hours, and substantial inhibition against Candida albicans (MIC 250 mg/mL, 93.01% inhibition) and Candida tropicalis (MIC 750 mg/mL, 90.23% inhibition), supporting T. biroi propolis as a promising natural source for fungal infections.

First Advisor

Llyrha Mae M. Capio, RMT, PhD

Download

Share

COinS