Date of Completion

2025

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Ehretia microphylla, Tsaang gubat, Anticoagulant, Thrombolytic, Streptokinase, Heparin, Phytochemicals, Prothrombin Time, Activated Partial Thromboplastin Time, Clot Lysis

Abstract

The study aimed to evaluate the in vitro anticoagulant and thrombolytic properties of Ehretia microphylla (tsaang gubat) ethanolic leaf extract using human blood samples. The extract was subjected to phytochemical screening and tested for its ability to prevent and dissolve blood clots through the assessment of Prothrombin Time (PT), Activated Partial Thromboplastin Time (aPTT), and clot lysis. Flavonoids, tannins, alkaloids, phenols, and saponins were identified in the extract. These are phytochemicals associated with anticoagulant and thrombolytic activities. Three concentrations of the extract (5%, 10%, and 20%) were tested for the anticoagulant activity, while another set of three concentrations (50%, 75%, and 100%) was used for thrombolytic testing. Results demonstrated a dose-dependent effect, with 20% concentration exhibiting the highest anticoagulant activity and the 100% concentration yielding the greatest clot lysis. The anticoagulant effect was evaluated against heparin (positive control), while thrombolytic activity was compared with streptokinase (positive control). Statistical analysis revealed significant differences between the effects of E. microphylla and the positive controls, suggesting that the extract possesses notable anticoagulant and thrombolytic activities. These findings support the potential use of E. microphylla as a natural therapeutic agent for thrombotic disorders.

First Advisor

Clarence Witty Mendoza-Fenol, RMT, MSMT, AHI (AMT)

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