Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Venom, Rice Paddy Herb, Bitter Gourd

Abstract

This study investigates the venom-neutralizing potential of crude extracts from two indigenous medicinal plants, Limnophila aromatica (Rice Paddy Herb) and Momordica charantia (Bitter Gourd), against the Naja philippinensis (Philippine cobra). The research identified the predominant bioactive compounds—particularly phenolics and flavonoids—present in the leaves of these plants and determined the optimal extract concentration required for effective venom neutralization. Using an indirect hemolytic assay, the study quantified the inhibition of phospholipase A₂ (PLA₂) activity, a key enzyme in cobra venom toxicity, and compared the efficacy of the plant extracts against the Purified Cobra Antivenom (PCAV) of equine origin. The results demonstrated significant venom-neutralizing activity, with both extracts exhibiting optimal inhibition at a concentration of 100 µg/µL; specifically, Momordica charantia reduced the hemolytic halo to approximately 14.9 mm and Limnophila aromatica to about 15.1 mm—values that, while slightly higher than the positive control (12.1 mm), are comparable to conventional antivenom treatments. These findings highlight the potential of locally available medicinal plants as alternative therapeutic agents for snakebite management, particularly in regions with limited access to commercial antivenoms. The study underscores the need to explore further plant-based antivenom therapies to develop cost-effective and sustainable solutions for cobra envenomation.

First Advisor

Jion P. Dimson, RMT, MSMLS

Download

Share

COinS