Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Lung Cancer

Abstract

Non-small cell lung cancer (NSCLC) accounts for 85% of lung cancer patients which encompasses adenocarcinoma, squamous cell carcinoma, and large cell carcinoma. There are five stages of NSCLC: stage 0, the early stages (I and II) and late stages (III and IV). Cancer development is associated with genetic predisposition, lifestyle, environmental exposure, and interrelationship with microbial composition. The study aims to comparatively assess the microbial populations of stage I and stage II NSCLC patients through metagenomic analysis of samples through in silico study. The researchers obtained databases from MGnify and employed QIIME2 and RStudio to generate comparative analyses between the two samples sets. The taxonomic analysis of stage I NSCLC resulted in the predominant phyla of Proteobacteria, Firmicutes, and Actinobacteria; abundant classes of Gammaproteobacteria, Betaproteobacteria, and Bacilli; predominant family are Comamonadaceae, Enterococcaceae, and Xanthomonadaceae; and predominant bacteria of Enterococcus, Stenotrophomonas, and Pseudomonas in the genus classification. The predominant phyla in stage II NSCLC are Proteobacteria, Firmicutes, and Actinobacteria; the abundant classes included Bacilli, Gammaproteobacteria, and Actinobacteria; predominant bacteria based on family are Pseudomonadaceae, Staphylococcaceae, and Lactobacillaceae; and the top three most prevalent bacteria in genus are Pseudomonas, Lactobacillus, and Staphylococcus. A Wilcoxon rank sum test was done to assess the bacterial diversity between the two stages and yielded a score of 800 and a p-value of 0.0000. This signifies a significant difference in the bacterial diversity of the stages of NSCLC. The study's findings suggest that microbial composition may influence the progression of tumor stages in non-small cell lung cancer.

First Advisor

Theo John M. Segismundo, RMT

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