Date of Completion
2024
Document Type
Thesis
Degree Name
Bachelor of Science in Medical Laboratory Science
Keywords
Waterborne parasites, swimming pools, polymerase chain reaction, chlorine, nitrate
Abstract
Going to swimming pools are one of the affordable recreational activities for many Filipinos. Nonetheless, the physicochemical characteristics of these pools might contribute to the presence of waterborne parasites such as Cryptosporidium parvum and Giardia lamblia. Various studies in the Philippines suggest the isolation of these parasites in local water sources, including these pools. Thus, this study aims to determine the presence of Cryptosporidium parvum and Giardia lamblia in the recreational pools of Dasmariñas, Cavite using a quantitative turbidimetric loop-mediated isothermal amplification (LAMP) and correlate it to the selected swimming pool’s physicochemical characteristics such as temperature, pH, total dissolved solids, turbidity, nitrate, and residual chlorine. The workflow of the study involved vacuum filtration of the collected water samples, extraction of the genomic DNA, analysis of the extracted DNA using UV-vis spectroscopy and gel electrophoresis, LAMP testing, and turbidimetric analysis of the LAMP. Results showed that the swimming pools maintained acceptable physicochemical characteristics, except for nitrate. Also, both Cryptosporidium parvum and Giardia lamblia are absent in all the swimming pools tested attributed to their strict adherence to sanitation protocols. Furthermore, there is no significant correlation between the presence of the parasites based on the turbidity of the LAMP and the physicochemical characteristics of the swimming pools, except for nitrate and turbidity of water samples for Giardia lamblia, potentially due to interfering chemicals in the water sample.
First Advisor
Johnmartin M. De Los Reyes
APA Citation
Chan, L. S., Cruz, T. B., De Leon, Z. A., Embate, L. N., Estrella, M. T., & Frani, M. V. (2024). Physicochemical analysis of selected recreational pools in Dasmariñas City, Cavite and detection of Cryptosporidium parvum and Giardia lamblia using loop-mediated isothermal amplification. [Bachelor's thesis, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/bsmls/201
