Title

Enhancement of antimicrobial activity of ciprofloxacin using lactobacillus gasseri ATCC 119992 cel lysate against escherichia coli

Date of Completion

2017

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Anti-Infective Agents, Ciprofloxacin, Escherichia coli

Abstract

This study used experimental research design. Using purposive sampling technique, escherichia coli was used as the test sample of the study and alctobacillus gasseri ATCC 19992 cell free extract as an enhancer of ciprofloxacin to test the lower MIC breakpoints. Each organism was tested three times to the same microorganism in order to eliminate biased results and to have an accurate finding. Modified microboth dilution method was used to evaluate the antimicrobial activity of ciproloxacin and its combination with lactobacillus gasseri ATCC 19992. For the cell lysate, pure isolate of lactobacillus gasseri ATCC 19992 of fresh culture in MRS broth was centrifuged in 5000 rpm for 5 minutes, then supernatant was discarded, then the pellet was washed with phosphate buffer and was vortexed to mix the buffer with the pellet thoroughly. The wash step procedure was repeated for 3 times. Sonication for 15-20 minutes was done to lyse the cell completely. The results of the study found out that the minimum inhibitory centration of ciprofloxacin against escherichia coli is 0.9 ug/ml. This MIC has been used and dispense with different contrition’s of lactobacillus gasseri ATCC 19992. it is done in order to know if there is significance or any enhancement produced by the combination.

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