Date of Completion
2023
Document Type
Thesis
Degree Name
Bachelor of Science in Medical Laboratory Science
Keywords
mangosteen, Salmonella typhimurium, phytochemical compounds, minimum inhibitory concentration, minimum bactericidal concentration
Abstract
Salmonella typhimurium, a serovar of Salmonella enterica, is a gram-negative bacillus which causes food poisoning and acute gastroenteritis (Fàbrega & Villa, 2013). Garcinia mangostana, commonly known as mangosteen, has phytochemical compounds such as xanthone which provide its antibacterial properties. Due to their emerging resistance to several antibiotics, the study aimed to determine its antibacterial activity through utilizing two-fold serial microdilution for the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and disc diffusion method. Moreover, phytochemical testing was performed in order to confirm the presence of alkaloid, triterpenoids, and flavonoids. Descriptive analysis was utilized for qualitative interpretation of results. The study determined that all concentrations of mangosteen pericarp extract (2 mg/dL-0.025 mg/dL) were not effective against S. typhimurium, showing presence of turbidity in MIC. In addition, there was also a significant difference between the zone of inhibition of antibiotic control and concentrations of mangosteen pericarp extract against S. typhimurium, resulting to 45 mm and 6mm, respectively due to presence of turbidity. Therefore, Garcinia mangostana Linn. pericarp has no antibacterial activity against S. typhimurium ATCC#13311.
First Advisor
Carolina B. Capili
APA Citation
Macaraeg, G. S., Maliksi, R. S., Mapanao, P. C., Mendoza, S. C., Morales, A. T., & Parungao, K. S. (2023). In vitro antibacterial activity of Garcinia mangostana linn. (Mangosteen) pericarp extract against salmonella typhimurium. [Bachelor's thesis, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/bsmls/182