Date of Completion

2023

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Klebsiella pneumoniae, Silver Nanoparticles, Biofilm, Minimum Biofilm Inhibitory Concentration, Minimum Biofilm Eradication Concentration

Abstract

Klebsiella pneumoniae is a gram-negative bacteria that form biofilms, which are clumps of cells immersed in a matrix that facilitate bacteria’s surface adhesion, sustenance, and resistance to antimicrobial compounds (Samreen, Roy, and Ahmad, 2022). Currently, it resists most antibacterial drugs which makes eradication difficult. Hence, the goal of this study is to: confirm the presence of green synthesized silver nanoparticles (AgNPs) from the leaves of Ananas comosus (Pineapple), determine the characteristics of the green synthesized AgNPs, determine the minimum biofilm inhibitory concentration (MBIC) and the minimum biofilm eradication concentration (MBEC) of the green synthesized AgNPs using pineapple leaf extract against K.pneumoniae, and determine the significant difference between the anti-biofilm activity of green synthesized AgNPs using Pineapple leaf extract and Ciprofloxacin. The green synthesis was done by powdering the leaves, boiling, filtering, and then adding the aqueous silver nitrate solution. This was followed by the confirmation of the AgNPs present through Ultraviolet-Visible Spectroscopy, their characterization by Scanning Electron Microscopy (SEM), the determination of anti-biofilm activity of the synthesized AgNPs by MBIC and MBEC, and the confirmation of the anti-biofilm activity through SEM. At the end of the study, the researchers concluded that the MBIC is at 0.0975% (v/v) while the MBEC is at 6.25% (v/v). The anti-biofilm activity of the AgNPs was also comparable to Ciprofloxacin. These findings suggest that the AgNPs extracted from pineapple leaves can potentially inhibit biofilm-forming properties of K.pneumoniae.

First Advisor

Jion P. Dimson

Download

Share

COinS